High-intensity exercise decreases muscle buffer capacity via a decrease in protein buffering in human skeletal muscle - Insep - Institut national du sport, de l'expertise et de la performance Accéder directement au contenu
Article Dans Une Revue Pflügers Archiv European Journal of Physiology Année : 2009

High-intensity exercise decreases muscle buffer capacity via a decrease in protein buffering in human skeletal muscle

Résumé

We have previously reported an acute decrease in muscle buffer capacity (βm in vitro) following high-intensity exercise. The aim of this study was to identify which muscle buffers are affected by acute exercise and the effects of exercise type and a training intervention on these changes. Whole muscle and non-protein βm in vitro were measured in male endurance athletes (VO 2max =59.8± 5.8 mL kg −1 min −1), and before and after training in male, team-sport athletes (VO 2max =55.6±5.5 mL kg −1 min −1). Biopsies were obtained at rest and immediately after either time-to-fatigue at 120% VO 2max (endurance athletes) or repeated sprints (team-sport athletes). High-intensity exercise was associated with a significant decrease in βm in vitro in endurance-trained males (146±9 to 138±7 mmol H + ·kg d.w. −1 ·pH −1), and in male team-sport athletes both before (139±9 to 131±7 mmol H + ·kg d.w. −1 ·pH −1) and after training (152±11 to 142±9 mmol H + ·kg d.w. −1 ·pH −1). There were no acute changes in non-protein buffering capacity. There was a significant increase in βm in vitro following training, but this did not alter the post-exercise decrease in βm in vitro. In conclusion, high-intensity exercise decreased βm in vitro independent of exercise type or an interval-training intervention; this was largely explained by a decrease in protein buffering. These findings have important implications when examining training-induced changes in βm in vitro. Resting and post-exercise muscle samples cannot be used interchangeably to determine βm in vitro , and researchers must ensure that post-training measurements of βm in vitro are not influenced by an acute decrease caused by the final training bout.
Fichier principal
Vignette du fichier
Bishop et al. Pflugher archieve 458 (2009).pdf (466.86 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01587033 , version 1 (13-09-2017)

Identifiants

Citer

David Bishop, Johann Edge, Alberto Mendez-Villanueva, Claire Thomas, Knut Schneiker. High-intensity exercise decreases muscle buffer capacity via a decrease in protein buffering in human skeletal muscle. Pflügers Archiv European Journal of Physiology, 2009, 458 (5), pp.929 - 936. ⟨10.1007/s00424-009-0673-z⟩. ⟨hal-01587033⟩
156 Consultations
1665 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More