Effect of intermittent hypoxic training on HIF gene expression
in human skeletal muscle and leukocytes

Claire Thomas; Remi Mounier; Vincent Pialoux; Belle Roels; Grégoire Millet; Jacques Mercier; Jean Couderc; Nicole Fellmann; Eric Clottes

Effect of intermittent hypoxic training on HIF gene expression in human skeletal muscle and leukocytes

Claire Thomas ^{1, 2} Remi Mounier ³ Vincent Pialoux ³ Belle Roels ^{4, 5} Grégoire Millet ^{6, 5} Jacques Mercier ^{2, 1} Jean Couderc ³ Nicole Fellmann ³ Eric Clottes ^{3, 7}

1 Muscles et pathologies

2 UM1 Médecine - Montpellier 1 - UFR de Médecine

3 Laboratoire de Physiologie-Biologie du Sport

4 School of Sport and Education

5 EuroMov - Euromov

6 Academy for Sports Excellence, Doha, Qatar

7 IPBS - Institut de pharmacologie et de biologie structurale

Abstract : Intermittent hypoxic exposure with exercise training is based on the assumption that brief exposure to hypoxia is suYcient to induce beneWcial muscular adaptations mediated via hypoxia-inducible transcription factors (HIF). We previously demonstrated (Mounier et al. Med Sci Sports Exerc 38:1410–1417, 2006) that leukocytes respond to hypoxia with a marked inter-individual variability in HIF-1 mRNA. This study compared the eVects of 3 weeks of intermittent hypoxic training on hif gene expression in both skeletal muscle and leukocytes. Male endurance athletes (n = 19) were divided into an Intermittent Hypoxic Exposure group (IHE) and a Normoxic Training group (NT) with each group following a similar 3-week exercise training program. After training, the amount of HIF-1 mRNA in muscle decreased only in IHE group (¡24.7%, P < 0.05) whereas it remained unchanged in leukocytes in both groups. The levels of vEGF121 and vEGF165 mRNA in skeletal muscle increased signiWcantly after training only in the NT group (+82.5%, P < 0.05 for vEGF121; +41.2%, P < 0.05 for vEGF165). In leukocytes, only the IHE group showed a signiWcant change in vEGF165 (¡28.2%, P < 0.05). The signiWcant decrease in HIF-1 mRNA in skeletal muscle after hypoxic training suggests that transcriptional and post-transcriptional regulations of the hif-1 gene are diVerent in muscle and leukocytes.

Keywords : HIF-1x mRNA Natural antisense Skeletal muscle Leukocyte

Type de document :

Article dans une revue

Domaine :

Sciences de l'Homme et Société / Sport

Sciences de l'Homme et Société / Sport / Physiologie du sport

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https://hal-insep.archives-ouvertes.fr/hal-01587180
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Soumis le : mercredi 13 septembre 2017 - 17:41:48
Dernière modification le : vendredi 10 janvier 2020 - 21:08:28

Effect of intermittent hypoxic training on HIF gene expression in human skeletal muscle and leukocytes

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Effect of intermittent hypoxic training on HIF gene expression in human skeletal muscle and leukocytes

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