Acute induction of uncoupling protein 1 by citrulline in cultured explants of white adipose tissue from lean and high-fat-diet-fed rats

Philippe Noirez (1, 2) , Nolwenn Joffin (3, 4) , Anne-Marie Jaubert (3, 5) , Jessica Bamba (3, 4) , Robert Barouki (4, 3) , Claude Forest (3, 4)

1 INSEP - Institut national du sport, de l'expertise et de la performance
2 IRMES - EA 7329 - Institut de recherche biomédicale et d’épidémiologie du sport
3 T3S - UMR_S 1124 - Toxicité environnementale, cibles thérapeutiques, signalisation cellulaire
4 UPD5 - Université Paris Descartes - Paris 5
5 UVSQ - Université de Versailles Saint-Quentin-en-Yvelines

A diet enriched with citrulline (CIT) reduces white adipose tissue (WAT) mass. We recently showed that CIT stimulated β-oxidation in rat WAT explants from young (2–4 months) but not old (25 months) rats. Here we show that both in old rats and high-fat-diet-fed young rats, uncoupling protein one (UCP1) mRNA and protein expressions were weaker than those in young control rats. Selectively in WAT from young rats, a 24h CIT treatment up-regulated expressions of UCP1, peroxisome proliferator-activated receptor-x (PPARx/, PPARy-coactivator-1-x and mitochondrialtranscription- factor-A whereas it down-regulated PPARy gene expression, whatever the diet. These results suggest that CIT induces a new metabolic status in WAT, with increased β-oxidation and uncoupling of respiratory chain, resulting in energy expenditure that favors fat mass reduction.

Domaines: Sport; Santé du sportif; Alimentation et Nutrition

Philippe Noirez, Nolwenn Joffin, Anne-Marie Jaubert, Jessica Bamba, Robert Barouki, et al.. Acute induction of uncoupling protein 1 by citrulline in cultured explants of white adipose tissue from lean and high-fat-diet-fed rats. Adipocyte, 2015, 4 (2), pp.129 - 134. ⟨10.4161/21623945.2014.989748⟩. ⟨hal-01772974⟩