Acute induction of uncoupling protein 1 by citrulline in cultured explants of white adipose tissue from lean and high-fat-diet-fed rats

Abstract : A diet enriched with citrulline (CIT) reduces white adipose tissue (WAT) mass. We recently showed that CIT stimulated β-oxidation in rat WAT explants from young (2–4 months) but not old (25 months) rats. Here we show that both in old rats and high-fat-diet-fed young rats, uncoupling protein one (UCP1) mRNA and protein expressions were weaker than those in young control rats. Selectively in WAT from young rats, a 24h CIT treatment up-regulated expressions of UCP1, peroxisome proliferator-activated receptor-x (PPARx/, PPARy-coactivator-1-x and mitochondrialtranscription- factor-A whereas it down-regulated PPARy gene expression, whatever the diet. These results suggest that CIT induces a new metabolic status in WAT, with increased β-oxidation and uncoupling of respiratory chain, resulting in energy expenditure that favors fat mass reduction.
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https://hal-insep.archives-ouvertes.fr/hal-01772974
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Soumis le : vendredi 20 avril 2018 - 16:33:21
Dernière modification le : jeudi 13 juin 2019 - 11:06:06

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Philippe Noirez, Nolwenn Joffin, Anne-Marie Jaubert, Jessica Bamba, Robert Barouki, et al.. Acute induction of uncoupling protein 1 by citrulline in cultured explants of white adipose tissue from lean and high-fat-diet-fed rats. Adipocyte, Taylor & Francis, 2015, 4 (2), pp.129 - 134. ⟨10.4161/21623945.2014.989748⟩. ⟨hal-01772974⟩

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